The Role of Sox2 in Lung Cancer Initiation and Progression
Abstract
We have made gradual progress in each aim. In aim 1, we have identified TA p63 as the dominant isoform upregulated by Sox2. We then performed a chromatin immunoprecipitation using a Sox2 antibody followed by sequencing that did not identify p63 as a direct target of Sox2. Despite this finding, the sequencing data demonstrate several intriguing targets that we have begun to study. These include the protein tyrosine phosphatase zeta 1 (Ptprz1) and connective tissue growth factor (CTGF). In addition for this aim, we have re-derived and bred a p63 conditional knockout mouse to our conditional Sox2-overexpressing mouse. Over the next year, these mice will document the importance of p63 for Sox2-induced oncogenesis. For aim 2, we have performed initial transplants but need to troubleshoot the protocol to add supportive fibroblasts. For aim 3, we are breeding mice to pure strains in order to identify modifier genes that incidence of tumors after Sox2 overexpression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 29, 2011
- Accession Number
- ADA603904
Entities
People
- Mark Onaitis
Organizations
- Duke University