Micro and Nano-mediated 3D Cardiac Tissue Engineering
Abstract
The project envisages to improve the care of battlefield-related cardiac injuries by providing novel methods to design and fabricate 3-D models of cardiac sub-components that would be critical in restoring the function of the heart. We report the integration of dielectrophoresis (DEP) with stereolithography (SL) apparatus for the spatial patterning of cells on custom made gold micro-electrodes. We showed the patterning and encapsulation of mouse embryonic stem cells and skeletal muscle myoblasts. A robust and flexible in vitro platform was developed for stem cell differentiation and tissue engineering, by mimicking elements of the native 3D in vivo cellular micro-environment. It was shown that a sequential drug delivery can be attained with the hydrogel created in this study, and that the sequential delivery of two complementary proangiogenic factors can significantly increase vascular density in a target tissue. To test hydrogel constructs in vivo we developed a mouse myocardial infarction model. We demonstrated several new capabilities of photonic crystal biosensors using photonic crystal enhanced microscopy (PCEM), enabling visualizing of dynamic cell attachment at much higher resolution and sensitivity for highly detailed quantification of attachment distribution within individual cells. This provides a novel tool for the study of cell attachment for various applications, including wound healing, cell culture optimization, stem cell differentiation, and cancer metastasis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2012
- Accession Number
- ADA604941
Entities
People
- Brian Cunningham
- Hyunjoon Kong
- Lawrence B. Schook
- Rashid Bashir
- Taher Saif
Organizations
- University of Illinois Urbana–Champaign