Induced Accelerated Aging in Induced Pluripotent Stem Cell Lines from Patients with Parkinson's Disease

Abstract

The overall goal of the study was to develop a cell culture system from patient-derived induced pluripotent stem cells that expresses genes that can accelerate the aging process to facilitate modeling of neurodegenerative diseases such as Parkinson s disease. The objective of this study was to introduce the truncated lamin A gene, progerin, into patient-derived induced pluripotent stem cells using a doxycycline (Dox)-inducible expression system and differentiate them into dopaminergic neurons with the goal to accelerate the aging process of these cells and to promote a PD-related phenotype thus establishing a disease-in-a-dish model for PD. During our study, a publication from Lorenz Studer showed a very similar approach with lentiviral constructs of truncated lamin A in iPSC-differentiated neurons (Miller et al. 2013). It shows that our hypothesis is correct and that the introduction of mutant forms of lamin A that can cause Hutchinson Gilford Progeria syndrome is accelerating the neurodegenerative phenotype in vitro.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2014
Accession Number
ADA605975

Entities

People

  • Birgitt Schuele

Tags

DTIC Thesaurus Topics

  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Coinfection
  • Culture Techniques
  • Diseases And Disorders
  • Gene Expression
  • Genes
  • Infection
  • Neurodegeneration
  • Neurodegenerative Diseases
  • Neurons
  • Parkinson'S Disease
  • Retroviridae Infections
  • Stem Cells
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Immunology and Pathology
  • Molecular Genetics
  • Neurodegenerative Parkinson's Disease and Rickettsial Disease handbook, including the data level of dopamine, BC, neurons, and PD.

Technology Areas

  • Biotechnology