NF1 Signal Transduction and Vascular Dysfunction

Abstract

We have made strong progress on each of the proposed Aims. We have successfully generated reagents for, and begun the evaluation of, several of the regulatory nodes that comprise the mTOR pathway including Rheb, Pten, Raptor, and Rictor. We have found that Rheb up- regulation weakly phenocopies NF1 loss, while PTEN loss shares many similar characteristics. We have found conditions to induce EndMT in primary endothelial cells. While data to date do not support the hypothesis that this is being modulated by the loss of NF1, this analysis is ongoing. Preliminary results suggest that the loss of NF1 may alter the balance of ALK receptor signaling and that the loss of ALK5 signaling can phenocopy the morphogenic defects seen with NF1 loss. Lastly we have successfully established the genetic lines required for our in vivo experiments and we are poised to begin the Cre-induction in the first experimental cohort.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2014
Accession Number
ADA605985

Entities

People

  • Kevin Pumiglia

Organizations

  • Albany Medical College

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Blood
  • Cardiovascular Diseases
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Demographic Cohorts
  • Endothelial Cells
  • Genetics
  • Growth Factors
  • Neoplasms
  • Neuromuscular Diseases
  • Peptide Growth Factors
  • Regulations
  • Vascular Endothelium

Fields of Study

  • Biology

Readers

  • Aquatic Ecology
  • Molecular and Cellular Biology
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology