Further Biodosimetry Investigations Using Murine Partial-body Irradiation Model
Abstract
This study evaluates both the effects of physical restraint and use of candidate biomarkers in a CD2F1 male mouse partial-body irradiation model for biological dosimetry diagnostic assays. Mice were irradiated (6-Gy, 250-kVp X ray) to 3/3rd (total body), 2/3rd (gut and torso), 1/3rd (gut only) and 0/3rd (sham) of total body. Blood was sampled for haematology and blood plasma proteomic biomarkers at 1 and 2 d after exposure. Increases in the body fraction exposed showed progressive decreases in lymphocyte counts and increases in the neutrophil-to-lymphocyte ratios with no significant differences in the neutrophil and platelet counts. The radioresponse for plasma biomarker Flt3L showed proportional increases; however, G-CSF and SAA levels exhibited dramatic and non-proportional increases in levels. Physical restraint at 1 d post-exposure increased lymphocyte counts and SAA, decreased neutrophil-to-lymphocyte ratio and Flt3L and showed no effects on neutrophil and platelet counts or G-CSF.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 21, 2014
- Accession Number
- ADA606218
Entities
People
- D. J. Sandgren
- G. B. Sigal
- N. I. Ossetrova
- Seungchan Kim
- V. Nagy
- William F. Blakely
Organizations
- Armed Forces Radiobiology Research Institute