Systemic and Local Vaccination against Breast Cancer with Minimum Autoimmune Sequelae
Abstract
Our goal is to eliminate the tumor by vaccination and local ablation to render long-term immune protection without excessive autoimmune sequelae. Complimenting this regimen is systemic modulation of natural/induced Treg (iTreg) and intratumoral expression of immune augmenting molecules. The two aims are to (1) Test the hypothesis that cryosurgery of cytokine enriched tumors amplifies Her-2 vaccine response, and (2) Test the hypothesis that disabling iTreg conversion enhances Her-2 immunity, not autoimmunity. In aim 1, we show that tumor destruction by cryoablation enables antigen presentation or manifestation of existing immunity. Treatment with CpG ODN immediately after cryoablation reduces local recurrence and improves systemic anti-tumor immunity. While pursuing Aim 2, we discovered that in addition to the loss of inducible Treg, TIEG1 deficiency also leads to increased mammary tumorigenesis in NeuT mice, indicating tumor suppressive activity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2013
- Accession Number
- ADA606390
Entities
People
- Wei-Zen Wei
Organizations
- Wayne State University