Epigenetic Control of Prostate Cancer Metastasis: Role of Runx2 Phosphorylation
Abstract
The goal of this project is to determine the role of ERK/MAP kinase phosphorylation of the RUNX2 transcription factor in the metastasis of prostate cancer cells. In the third budget year, we achieved the following: a. Generation of retrovirus and lentivirus vectors expressing WT RUNX2 and S301A, S319A phosphorylation-deficient RUNX2 and S301E, S319E phosphomimetic Runx2 mutants. Isolation of stable PC3 and LnCaP cell lines expressing WT and mutant RUNX2. b. Demonstration that phosphorylation-deficient RUNX2 has reduced ability to stimulate in vivo tumor formation when PCa cells are implanted into immunodeficient mice. These results continue to support our overall hypothesis that RUNX2 phosphorylation is a critical determinant of tumorogenicity and metastasis of prostate tumor cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2014
- Accession Number
- ADA606599
Entities
People
- Chunxi Ge
- Evan T Keller
- Laurie K. Mccauley
- Renny T Franceschi
- Russell S. Taichman
- Silvana Papagerakis
- Yan Li
Organizations
- University of Michigan