Rapid Parallel Screening for Strain Optimization

Abstract

We have identified and characterized a biosensor for auxin and constructed the corresponding biosynthetic cassette. The system is shown in figure 1. Three variants have been made so that we can select on ampicillin, chloramphenicol, or kanamycin. The system also contains a co-transcribed GFP marker for quantitation. After full validation of genotypes we will begin characterizing auxin production and the associated readout provided by the biosensor cassette. Mutagenesis of the host and increased auxin production will follow.

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Document Details

Document Type
Technical Report
Publication Date
Aug 15, 2014
Accession Number
ADA607659

Entities

People

  • Chuck Merryman
  • Tony Yee

Organizations

  • J. Craig Venter Institute

Tags

Communities of Interest

  • Biomedical
  • Materials and Manufacturing Processes
  • Sensors

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Bacteria
  • Biology
  • Biosensors
  • Cassettes
  • Contracts
  • Department Of Defense
  • Detectors
  • Genes
  • Genetic Phenomena
  • Genetic Structures
  • Genetics
  • Genome
  • Microorganisms
  • Optimization
  • Production
  • Transcription Factors

Readers

  • Microbial Pathology
  • Molecular Biology and Genetics
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology