Imaging Prostate Cancer with Positron Emission Tomography
Abstract
Tumors associated fibroblasts (TAFs) represent a major component of a PCa tumor, and play a critical role in tumor development. The purpose of this proposal is to utilize fibroblast activation protein alpha (FAP) expression on TAFs within the tumor stroma for the diagnostic imaging of PCa using novel radiopharmaceuticals and innovative multimodal imaging platforms. The scope of this proposal is to develop peptide based radiopharmaceuticals and evaluate them as PET imaging agents in preclinical animal models of prostate cancer, which demonstrate TAF involvement. This progress report describes the completed synthesis of these peptide ligands, their radiosynthesis with copper-64 and an evaluation of their stability in human serum. A second goal achieved during the current funding period was the development of fibroblast based cell lines, which stably express eGFP and FAP. Ongoing experiments are focused on the in vitro and in vivo evaluation of each radiopharmaceutical and on understanding the growth characteristics of each transfected cell line in vivo.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2014
- Accession Number
- ADA608223
Entities
People
- Thaddeus J Wadas
Organizations
- Wake Forest University