The Importance of Neurogenic Inflammation in Blast-Induced Neurotrauma

Abstract

This study investigates the role of blood-borne immune cells as important mediators of damage and neuropathology resulting from blast-induced neurotrauma (BINT). We hypothesized that macrophages, along with their secreted cytokines and chemokines from the periphery, migrate via blood and infiltrate the CNS where they contribute to neuronal damage caused by activated microglia both in acute and chronic injury phases of BINT. We have used MRI and molecular techniques in mice with mild/moderate blast injury generated in a compressed helium-driven shock tube, as well as measurements of motor performance and behavioral assessment, observed temporally over 1 month. Groups of animals with mild/moderate BINT are imaged (MRI) and validated by immunocytochemistry, to visualize potential macrophage infiltration; blood-brain barrier (BBB) disturbance; reactive gliosis; or astrocyte activation. We show that a single exposure to mild/moderate blast induces both acute and chronic glial activation, levels of cytokines/chemokines, and motor impairment.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2014
Accession Number
ADA609244

Entities

People

  • Brock Wester
  • Chris Bradburne
  • Michele Schaefer
  • Nathan Boggs

Organizations

  • Johns Hopkins University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Blood
  • Brain
  • Brain Injuries
  • Cells
  • Chemistry
  • Medical Personnel
  • Neurodegeneration
  • Neurosciences
  • Proteins
  • Wounds And Injuries

Fields of Study

  • Medicine

Readers

  • Immunology and Pathology
  • Neurotrauma and Rehabilitation Medicine.