Identification of Epigenetic Changes in Prostate Cancer using Induced Pluripotent Stem Cells

Abstract

Infection of human prostatic epithelial cells with lentiviruses expressing Oct4, Sox2, Klf4 and c-myc generated induced pluripotent stem cells (iPSCs). Comparison of gene and protein expression of these prostatic iPSCs and embryonic stem cells (ESCs) revealed similarities but also some differences. Prostate iPSCs could be induced into endodermal, ectodermal, and mesodermal lineages, confirming pluripotency. In response to inductive factors, prostate iPSCs differentiated into basal and secretory prostatic epithelial cells in vitro and in vivo. Methylation profiling revealed epigenetic changes that occur temporally as pluripotent cells convert first to basal cells, then transit amplifying cells expressing androgen receptor (AR) I and finally secretory cells expressing prostatespecific antigen (PSA). Prostatic iPSCs provide a novel model for delineating the epigenetic changes involved in regulation of lineage differentiation of the prostatic epithelium.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2014
Accession Number
ADA609267

Entities

People

  • Donna M. Peehl

Organizations

  • Stanford University

Tags

DTIC Thesaurus Topics

  • Androgen Receptors
  • Androgens
  • Antigens
  • Biological Sciences
  • Cells
  • Chemical Reactions
  • Chemistry
  • Culture Techniques
  • Embryos
  • Epithelial Cells
  • Genetics
  • Neoplasms
  • Peptide Growth Factors
  • Prostate Cancer
  • Proteins
  • Stem Cells
  • Tissues

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular and Cellular Biology
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology