Brain Region and Cell Type Transcripts for Informative Diagnostics

Abstract

Our study will provide a set of candidate markers that can be used not only for the diagnosis of brain trauma, such as traumatic exposure to explosions, but also diseases such as brain cancer, including glioblastoma, and neural degenerative diseases, such as Alzheimer s disease. This year we used our EigenBrain approach to identify specific regions that are highly expressed in each cell type. In addition, we discovered a strong candidate set of brain-specific and cell-type specific transcripts. Moreover, we applied the region-based clustering method to the in situ hybridization of cell type specific genes. This revealed dramatic spatial consistency of neuron-specific genes, sufficient to recapitulate most anatomical brain regions from gene expression alone. We also analyzed brain RNAseq data to measure transcripts present in both human and mouse brains. This allowed us to detect the presence of many brain-specific genes (798 transcripts) that improved our candidate selection.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2010
Accession Number
ADA609543

Entities

People

  • Leroy E. Hood

Organizations

  • Institute for Systems Biology

Tags

DTIC Thesaurus Topics

  • Alzheimer Disease
  • Biomedical Research
  • Blood-Brain Barrier
  • Brain
  • Cell Line
  • Cells
  • Central Nervous System
  • Cerebral Cortex
  • Computational Biology
  • Data Sets
  • Department Of Defense
  • Diseases And Disorders
  • Gene Expression
  • Identification
  • Image Registration
  • Nervous System
  • Supervised Machine Learning

Fields of Study

  • Biology

Readers

  • Computer Vision.
  • Molecular Genetics
  • Neuroscience