DNase 1 Retains Endodeoxyribonuclease Activity Following Gold Nanocluster Synthesis

Abstract

Here we present the synthesis of the enzyme DNase 1 stabilized gold nanoclusters (DNase 1:AuNCs) with core size consisting of either 8 or 25 atoms. The DNase 1:Au8NCs exhibit blue fluorescence whereas the DNase 1:Au25NCs are red emitting. In addition to the intense fluorescence emission, the synthesized DNase 1:AuNC hybrid retains the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 microg/mL. The DNase 1:AuNCs could be conveniently employed as efficient and fast sensors to augment the current time-consuming DNA contamination analysis techniques.

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Document Details

Document Type
Technical Report
Publication Date
Jul 04, 2014
Accession Number
ADA610451

Entities

People

  • Abby L. West
  • Daniel P Cole
  • Mark H Griep
  • Shashi P. Karna

Organizations

  • United States Army Research Laboratory

Tags

Communities of Interest

  • Energy and Power Technologies
  • Weapons Technologies

DTIC Thesaurus Topics

  • Analytical Chemistry
  • Biomolecules
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Electron Microscopy
  • Emission
  • Emission Spectra
  • Enzymes
  • Hybrid Systems
  • Materials
  • Metals
  • Nanoparticles
  • Physical Properties
  • Proteins
  • Spectroscopy

Readers

  • Nanoscale Plasmonic Nanotechnology