An In Vivo shRNA-Drug Screen to Identify Novel Targeted Therapy Combinations for KRAS Mutant Cancers

Abstract

The primary shRNA-drug screen utilizing PDAC cell lines has been completed. The top 100 gene targets that specifically cooperate with MEK inhibitors were prioritized using the statistical RNAi gene enrichment ranking (RIGER) algorithm, which considers all shRNAs against a specific gene as a hairpin set . Construction of the inducible shRNA mini-pool based on the top 100 gene hits from the primary screen is currently underway. Upon completion, this shRNA mini-pool will be utilized to perform the secondary shRNA-drug screen in an orthotopic mouse pancreatic cancer model as originally proposed.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2013
Accession Number
ADA610560

Entities

People

  • Ryan B. Corcoran

Organizations

  • Massachusetts General Hospital

Tags

DTIC Thesaurus Topics

  • Algorithms
  • Anatomy
  • Biological Sciences
  • Biomedical Research
  • Cancer
  • Cell Line
  • Cells
  • Clinical Trials
  • Combination Therapy
  • Construction
  • Department Of Defense
  • Electronic Mail
  • Health Services
  • Inhibitors
  • Neoplasms
  • Therapy
  • Xenografts

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Oncology