Assessing the Mechanisms of MDS and its Transformation to Leukemia in a Novel Humanized Mouse
Abstract
The overall goal of this project is to develop a model for the study of myelodysplasia (MDS), an acquired bone marrow failure syndrome in the aging population and in cancer survivors. MDS is inherently difficult to study. MDS stem cells cannot be grown in culture and in vivo models are thus the gold standard. However, MDS stem cells are diseased and fail to efficiently engraft in current immunodeficient mouse models. We have optimized engraftment of normal adult HSPC into MISTRG. We have determined optimal cell preparation, cell number, preengraftment irradiation dose, transplantation route and recipient age. We have optimized the analysis of engrafted humanized mice. We have successfully transplanted primary MDS bone marrow cells into MISTRG mice with successful engraftment and replication of the donor disease status. MISTRG humanized mice tolerate chemotherapy and thus represent an ideal model to study disease progression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2014
- Accession Number
- ADA610689
Entities
People
- Stephanie Halene
Organizations
- Yale University