Characterizing and Targeting Replication Stress Response Defects in Breast Cancer

Abstract

During the fourth year of this project, we have made significant progress in several of our proposed tasks. We found that TUSC4 is a potent tumor suppressor gene in breast cancer; its deficiency alone can sufficiently transform normal breast epithelial cells and promote tumor growth in mice. We further demonstrated that mechanistically TUSC4 protects BRCA1 protein from degradation by interfering its binding to Herc2 E3 ligase. In addition, we have validated the in vivo effects of MEK inhibitor, AZD6244 on targeting RSRD breast cancer cells in a xenograft mouse model. Finally, we have successfully optimized and reduced the size of hallow gold nanoparticle (HAuNS) and modified a functional group of AZD6244 so it can be conjugated to HAuNS.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2014
Accession Number
ADA611097

Entities

People

  • Chun-jen Lin
  • Hui Dai
  • Ju-Seog Lee
  • Lili Gong
  • Shiaw-Yih Lin

Organizations

  • The University of Texas MD Anderson Cancer Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Clinical Trials
  • Epithelial Cells
  • Inhibitors
  • Membrane Proteins
  • Metallic Nanoparticles
  • Nanoparticles
  • Neoplasms
  • Particle Size
  • Particles
  • Proteins
  • Suppressors
  • Targeting
  • Xenografts

Fields of Study

  • Biology

Readers

  • Electrochemical Surface Science
  • Molecular Biology and Genetics
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech