Identification of the Elusive Mammalian Enzyme Phosphatidylcholine-Specific Phospholipase C
Abstract
The long-term purpose of the present proposal aims at establishing the role of the elusive mammalian protein, phosphatidycholine-specific phospholipase C (PC-PLC) in the inflammatory processes involved in progression of rheumatoid arthritis (RA). Thus, the main scopes of this proposal are: 1. to identify the PC-PLC gene and protein; and 2. to test PC-PLC involvement in production of TNF alpha by monocytes. Major findings. In the first year of funding, we have mostly worked toward the identification of PC-PLC (Aim 1). Based on results published by other groups, we proposed to compare transcriptome analysis between two PC-PLC activating conditions (treatment with LPS and oxidized LDLs) to identify candidate PC-PLC mRNAs. While we found that the published activation of human PC-PLC by LPS in HUVEC cells was due to Phospholipase D activity and not PC-PLC, we identified another condition (high serum) that leads to reproducible and specific activation of PC-PLC and that can be substituted to LPS in the transcriptome analysis. Moreover, we found that most of activated PC-PLC is in the cytosol with important implications for both the analysis of elevated transcripts and the possibility of chromatographic purification of PC-PLC.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2014
- Accession Number
- ADA611640
Entities
People
- Chiara Luberto
Organizations
- State University of New York