A Specific Screening Strategy to Reduce Prostate Cancer Mortality

Abstract

Here, we report the use of a cancer-specific promoter, inhibition of differentiation-1 (Id1), to drive a dual-reporter system (Ad5/3- Id1-SEAP-Id1-mCherry) designed for detection of prostate cancer using a blood-based reporter-secreted embryonic alkaline phosphatase (SEAP) and tumor visualization using a fluorescent reporter protein, mCherry. In human prostate tumors, Id1 levels are correlated with increased Gleason grade and disease progression. To evaluate in vivo performance, flank tumors were grown in athymic male mice using three prostate cancer cell lines. Following intra-tumoral injection of the vector, tumors formed by cells with high Id1 had the greatest reporter expression. Interestingly, tumors with the lowest levels of Id1 and reporter expression produced the greatest amounts of PSA. These data support the use of Ad5/3-Id1-SEAP-Id1-mCherry as a predictor of prostate cancer malignancy and as a strategy for tumor localization. This strategy would assist clinicians in the detection and treatment of prostate cancer and ultimately reduce the mortality associated with this disease.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2014
Accession Number
ADA611990

Entities

People

  • Kurt R. Zinn

Organizations

  • University of Alabama

Tags

DTIC Thesaurus Topics

  • Blood
  • Cancer
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Confocal Microscopy
  • Detection
  • Gene Therapy
  • Health Services
  • Medical Personnel
  • Tissues

Fields of Study

  • Medicine

Readers

  • Oncology (Cancer Research).
  • Prostate Cancer Biology.