Portable Low-Volume Therapy for Severe Blood Loss
Abstract
In our Year 2 Annual Report, we reported experiments described in Specific Aim 2. These included determining if outcomes from hemorrhagic shock in rats can be improved by combining BHB/M with the proven hypothermia-promoting adjunct 3- iodothyronamine (T1AM). This year, we explored the feasibility of administering a larger volume of a lower molarity BHB/M to hemorrhagically shocked rats as described in Specific Aim 3. Results from these experiments are described in this Annual Report for Year 3. In summary we made the following conclusions: 1.)Melatonin provides therapeutic effects at very low concentrations. This was shown by the low histopathological score observed when administering a solution containing melatonin at a concentration 10-6-fold lower than that published by Klein et al [3]. 2.)An isotonic form of BHB/M is not efficient for sustaining survival when compared to LR. Lactated Ringers (LR) administration is more efficient at sustaining survival after 60% blood loss both at 24 hours and 10 days after surgery than any other treatment. LR provides essential mineral constituents of blood that isotonic BHB/M does not. 3.) 10-day survivors are on their way to full recovery. The low histopathological scores for all the treatments administered suggest that if an animal has made it to 10 days it is not likely to develop multiple organ failure and die after the endpoint of our experiment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2014
- Accession Number
- ADA612365
Entities
People
- Cecilia E. Rodriguez
- Lester R Drewes
- Matthew T Andrews
Organizations
- University of Minnesota Duluth