The Role of Metastasis-Associated Proteases and Peptides in Prostate Cancer

Abstract

In this project, we are testing the hypothesis that metastatic prostate cancer cells secrete a common signature of neuroactive peptides. We acquired two new state-of-the-art mass spectrometers and transferred and updated our existing workflows to the new instruments. Further, we established three complementary quantitation and identification MS-based proteomics workflows that allow us to interrogate the secretome of prostate cancers at unprecedented levels. We now have the most advanced setup to tackle the complex repertoire of molecular factors that are secreted by cancer cells and associated stromal cells. Ultimately, we aim to identify proteases and peptides that are responsible for tumor-induced pain and sensory neuropathy. By interfering with the generation of neuroactive peptides through specific protease inhibition, we aim to develop novel mechanism-based therapies for cancer pain.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2014
Accession Number
ADA612371

Entities

People

  • Markus Hardt

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Cells
  • Instrumentation
  • Mass Spectrometers
  • Mass Spectrometry
  • Metastasis
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Proteomics
  • Spectrometers
  • Spectrometry
  • Stromal Cells
  • Surface Plasmon Resonance

Fields of Study

  • Biology

Readers

  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.
  • Research Science/Academic Research

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech