Compound 49b Reduces Inflammatory Markers and Apoptosis after Ocular Blast Injury
Abstract
In year 2 of this project, we determined whether Compound 49b, a novel beta-adrenergic receptor agonist, prevented increased inflammatory and apoptosis proteins in insulin-like growth factor binding protein 3 (IGFBP-3) knockout (KO) mice after exposure to ocular blast. Eyes from IGFBP-3 KO mice were exposed to a blast of air from a paintball gun at 26psi. Eyes were collected at 4, 24, and 72 hours after blast exposure. In a subset of mice, Compound 49b eye drops (1mM dose) were applied within 4 hours, 24 hours, or 72 hours after blast. Three days after exposure to blast, all mice were sacrificed. One eye was used for protein analyses of TNF , IL-1 , Bax, Bcl-xL, caspase 3, and cytochrome C. We found that ocular exposure to 26psi of air pressure led to a significant increase in both inflammatory and apoptotic proteins. When Compound 49b was applied within 4 or 24 hours after blast, it mitigated the increase in inflammatory and apoptotic proteins. Ocular blast produces a significant increase in inflammatory and apoptotic proteins in the retina, specifically in retinal ganglion cells. These proteins are reduced after treatment with a topical beta-adrenergic receptor agonist. This data suggests local application of beta-adrenergic receptor agonists may be protective in ocular blast.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2014
- Accession Number
- ADA612557
Entities
People
- Jena J. Steinle
Organizations
- University of Tennessee