Studies of the Effects of Perfluorocarbon Emulsions on Platelet Number and Function in Models of Critical Battlefield Injury
Abstract
Perfluorocarbon emulsions (PFCs) can treat traumatic injuries (traumatic brain injury (TBI), hemorrhagic shock and burns) by enhanced delivery of oxygen. A class-based side effect of PFC (day 2-5 after infusion in 30-50%) may be thrombocytopenia (TCYP). The mechanism is inadequately investigated but is caused by reduced production or enhanced clearance (partial activation) of platelets (Plts). The United States Food and Drug Administration (FDA) requests investigation of the phenomenon to exclude Plt inflammatory/embolic safety risks. In phase one of the study, healthy juvenile sheep were used with a top load intravenous infusion of either PFC (Oxygent, n=7), Hespan (n=6), or na ve/saline control (n=6). Venous blood was sampled before the treatment (baseline) and at 0 minute, 3 and 24 hours, 4 and 7 days after treatment. Blood samples were measured for coagulation parameters including platelet count, fibrinogen, thrombin, CD62p etc. The results showed that the sheep s platelet count and fibrinogen level were not significantly reduced after PFC top-loaded for the 7 survival days. Platelet activation assay (CD62p) also showed no increase compared with control groups (na ve & Hespan). Morphologically, platelet activation was not significantly increased compared with baseline or controls. Therefore, treatment with Oxygent in healthy sheep did not cause massive or severe coagulopathy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2014
- Accession Number
- ADA612698
Entities
People
- Bruce D Spiess
Organizations
- Virginia Commonwealth University