Role of IKK-alpha in the EGFR Signaling Regulation
Abstract
Overexpression of EGFR is frequently linked to more aggressive tumor behavior, including increased proliferation, metastasis, and therapeutic resistance. Here, we identified a molecular linkage between IKK(alpha) and EGFR signaling in breast cancer cells. Inhibition of IKKs activity elevates EGFR tyrosine phosphorylation. In addition, IKK(alpha) forms a specific interaction with EGFR in Golgi apparatus and catalyzes EGFR S1026 phosphorylation. We found that EGFR S1026A possess a stronger tumorgenesis phenotype compare with wild type EGFR suggesting a negative regulation of IKK(alpha) in EGFR signaling. In agreement with an earlier finding where conditional ablation of IKK(alpha) in the mice keratinocytes elevates the autocrine loop of EGFR, our results further provide a potent role of IKK(alpha) kinase activity in preservation of EGFR activity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2014
- Accession Number
- ADA612726
Entities
People
- Chia-wei Li
Organizations
- The University of Texas MD Anderson Cancer Center