Toward an Inexpensive Test for Vitamin D Levels in Blood

Abstract

The objective of the research is to test the feasibility of a vitamin D biosensor based on a selective redox enzyme immobilized onto an electrode. In long-term, this will make it possible to create a technology akin to commercial glucose sensors for in-clinic use as part of routine examinations by both reducing the cost of testing vitamin D levels and reducing the time for testing to less than a few minutes. For this purpose, a synthetic gene for recombinant human CYP27B1 (the enzyme which is involved in vitamin D metabolism) was designed. The enzyme was expressed in E. coli and the activity of this enzyme was verified spectrophotometrically. Finally, a synthetic redox mediator (phenosafranine) was found to be successful in mediating electron transfer between electrode and CYP27B1. This is important for introducing a way to electrochemically measure 25(OH)D (circulating form of vitamin D in blood) levels.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2013
Accession Number
ADA612772

Entities

People

  • Alan C West
  • Harun F. Ozbakir
  • Linda Linday
  • Scott Banta
  • Sudipta Majumdar

Organizations

  • Columbia University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Aminolevulinic Acid
  • Biomedical Research
  • Cells
  • Cellular Structures
  • Chemical Compounds
  • Chemistry
  • Detection
  • Electrodes
  • Electron Transfer
  • Electrons
  • Glycerols
  • New York
  • Oxidation
  • Photoelectrochemical Cells
  • Proteins
  • Substrates
  • Vitamin D

Readers

  • Analytical Chemistry
  • Electrochemical Surface Science
  • Systems Analysis and Design

Technology Areas

  • Biotechnology
  • Microelectronics
  • Microelectronics - Microelectromechanical Systems