In Vivo 18-FDG/18-Choline-Mediated Cerenkov Radiation Energy Transfer (CRET) Multiplexed Optical Imaging for Human Prostate Carcinoma Detection and Staging

Abstract

Prostate cancer is treatable in its earliest stages, although treatment options for advanced forms are limited. Therefore, more sensitive means of early prostate cancer detection and new prostate cancer therapies are needed. Two novel biomarkers are proposed to associate with prostate cancer progression: the Thomsen-Friedenreich disaccharide (TF) antigen and the ErbB-2/ErbB-3 heterodimer (ErbB2/3). The objective of this proposal is to examine whether internal illumination via 18F-fluorocholine Cerenkov radiation energy transfer (CRET) coupled with TF- and ErbB2/3- molecularly targeted near-infrared (NIR) QDs can be used to detect prostate cancer. We have shown that ErbB2/ErbB3 dimerization is hergulin mediated and upregulated in castrated mice bearing MDAPCa- 2b human prostate cancer xenografts. We have selected peptides from bacteriophage display libraries that target TF and ErbB2/ErbB3. The peptides have been attached to QDs and are being used to detect prostate cancer cell lines that express TF, ErbB2/ErbB3 and various stages of cancer.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2014
Accession Number
ADA612850

Entities

People

  • Susan L. Deutscher

Organizations

  • University of Missouri

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Bacteriophages
  • Biological Markers
  • Cell Line
  • Cells
  • Cerenkov Radiation
  • Detection
  • Diagnostic Imaging
  • Disaccharides
  • Energy
  • Energy Transfer
  • Neoplasms
  • Prostate Cancer
  • Proteins
  • Quantum Dots
  • Radiation
  • Tissues
  • Xenografts

Readers

  • Molecular and Cellular Biochemistry
  • Optical Physics and Photonics.
  • Prostate Cancer Biology.