White Matter Glial Pathology in Autism

Abstract

Methods used to directly study the autism brain include brain imaging in living patients and pathology studies using postmortem brain tissues from deceased autism spectrum disorder (ASD) donors. These methods typically focus on brain regions as a whole with little regard to the underlying cellular complexity. While informative, these approaches do not provide information about the specific brain cells affected and also have not been successful in revealing the underlying cause of autism. This project uses innovative methods and a novel approach to investigate the pathology of the brain autism spectrum disorder (ASD). This research employed laser capture microdissection to isolate specific cell populations from carefully defined and specific brain regions from ASD and typically developing control brains. These cells were used to interrogate gene expression abnormalities that may underlie biological mechanisms that contribute behavioral abnormalities of ASD. By examining the ASD brain at the level of its most basic component, the cell, we seek to reveal a potentially unifying cellular pathology of the ASD brain that could be used for the development of therapeutic alternatives for ASD patients.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2014
Accession Number
ADA613167

Entities

People

  • Gregory A. Ordway
  • Jessica D. Crawford
  • Michelle J Chandley

Organizations

  • East Tennessee State University

Tags

DTIC Thesaurus Topics

  • Abnormalities
  • Alzheimer Disease
  • Amplification
  • Astrocytes
  • Autism
  • Brain
  • Depression
  • Diseases And Disorders
  • Gene Expression
  • Genetic Phenomena
  • Medical Personnel
  • Pathology
  • Professional Development
  • Quality Control
  • Spectra
  • Students
  • Transcriptomics

Fields of Study

  • Biology

Readers

  • Child and Adolescent Substance Abuse Science in Autism Spectrum Disorders.
  • Molecular and genetic basis of cancer.
  • Neuroscience

Technology Areas

  • Directed Energy