Advancing the Capabilities of an Authentic Ex Vivo Model of Primary Human Prostate Cancer
Abstract
We have begun to optimize and characterize the novel tissue slice culture (TSC) model for benign and primary human prostate cancer (PCa) of different Gleason grades. Under optimized culture conditions, TSCs authentically recapitulate the cellular and molecular heterogeneity of the human tissue for up to 5 days in culture. They also exhibit androgen-dependence and can be used to evaluate drug efficacy and specificity in vitro. Results suggest that the TSC system addresses several deficiencies of current pre-clinical models and is a more authentic and clinically relevant model for human PCa. We have begun to combine the TSC model with the in vivo tumorgraft model in which human tissue slices are implanted under the renal capsules of immune-deficient mice. Culturing tissue slices for 3-4 days pre-implantation not only relieves time constraints, but it also provides an opportunity for experimental manipulation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2014
- Accession Number
- ADA613178
Entities
People
- Sophia L. Maund
Organizations
- Stanford University