Role of Mitochondrial Inheritance on Prostate Cancer Outcome in African-American Men
Abstract
We are examining the hypothesis that mitochondrial inheritance plays a significant role in aggressiveness of prostate cancer in African Americans. In the first year of the project we identified 2,000 noncancerous tissues samples from African American men with prostate cancer and we have extracted DNA from ~ 1,500 of them to date. We have validated a robust new DNA sequencing technique developed by our collaborator using single amplicon long-range PCR that permits deep coverage (10,000-20,000X on average) of the mitochondrial genome. We have sequenced 652 samples fully thus far and FFPE samples are now in the pipeline. Mapping of DNA variants in our sequenced genomes to mitochondrial genes has begun and the initial data is presented here. After months of testing, we determined that the ethidium bromide methodology for generating prostate cancer cell line cybrids was not effective and we have instead decided to use the Rhodamine-6-G procedure. Thus far PNT1A cybrid cell lines have been generated and we are actively characterizing these cells. PC3 cells have been more difficult, but we continue to work on this cell line.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2014
- Accession Number
- ADA613183
Entities
People
- Martha Mims
Organizations
- Baylor College of Medicine