Improving Joint Function Using Photochemical Hydrogels for Articular Surface Repair
Abstract
The goal of our research is to introduce a novel means to regenerate the articular cartilage and restore normal function of the joint. A strategy that can generate durable hyaline articular cartilage, which will be predominantly type II collagen, and is capable of integrating with the surrounding cartilage matrix (without fissures) could improve the long-term outcome of joint surface repair. Key findings are isolation of MSCs and stimulation towards osteogenesis in vitro on an octacalcium phosphate scaffold showing cells populated the scaffold and calcium deposits demonstrated with von Kossa stains; 2) A degradable form of photochemically crosslinked PEG norbomene gel was formulated and growth factors (TGF beta) tethered to the polymer showing peri-cellular cartilage matrix around the encapsulated swine chondrocytes; 3) Formation of new cartilage matrix was demonstrated in vivo in mice using photochemically crosslinked gels and swine articular chondrocytes 4) Chondrocytes encapsulated in photochemically crosslinked hydrogels can survive the crosslinking and implantation process as shown in swine 5) Bone marrow derived mesenchymal stem cells from swine were encapsulated in alginate, stimulated with chondrogenic growth factors, and showed in vivo matrix productions
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2014
- Accession Number
- ADA613491
Entities
People
- Mark A. Randolph
- Thomas J. Gill
Organizations
- Massachusetts General Hospital