Tribosupplementation with Lubricin in Prevention of Post-Traumatic Arthritis
Abstract
Full length recombinant human lubricin (rhPRG4) maximally reduced friction between apposed explants of bovine articular cartilage. Secretion levels exceeded 1 mg/ml when expressed by transfected CHO-M cells. This level of expression is commercially scalable and will thus enable production of a GMP protein for clinical use. A purification bioprocess utilizing anion exchange, hydroxyapatite and a hydrophobic exchange media resins achieves a high level of purity. Explants of bovine articular cartilage cultured and incubated with IL-1 followed by friction testing showed higher friction than explants not receiving IL-1. The rhPRG4 restored low friction and qualitatively restored native PRG4 expression as determined by immunohistochemistry. Cartilage from the femoral condyle shows variations in IL-1 induced elevated friction. Explants from the weight bearing region show a significant response to exogenous rhPRG4 in friction reduction from this simulated inflammatory environment. A large animal trial is now planned to test pre-GMP equivalent rhPRG4 in restoring chondroprotection in the ACL-injured joint.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2014
- Accession Number
- ADA613549
Entities
People
- Braden C Fleming
- Gregory Jay
Organizations
- Rhode Island Hospital