Assessment of Nanobiotechnology-Targeted siRNA Designed to Inhibit NF-KappaB Classical and Alternative Signaling in Breast Tumor Macrophages
Abstract
Macrophages have been proposed as a potential target for manipulation of the microenvironment in breast cancer because they are potent effectors of the immune system. NF kappaB (NF B) signaling in macrophages contributes to their impact during breast tumorigenesis. Thus, macrophage targeted modulation of NF B has potential as a novel therapeutic approach for breast cancer. NF B signaling is mediated via two major pathways; the canonical/classical pathway and the alternative pathway. Our strategy is designed to develop a nanobiotechnology-based method to target siRNA designed to inhibit NF B classical and alternative signaling specifically to tumor associated macrophages to modulate the tumor microenvironment and to test the therapeutic potential of this approach. In this highly collaborative stufy, we have synthesized and characterized in vitro both mannosylated and untargeted nanoparticles. We have compared the efficiency of transfection of bone marrow derived.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2014
- Accession Number
- ADA613613
Entities
People
- Fiona E. Yull
Organizations
- Vanderbilt University