Conjugation of the Dark Quencher QSY 7 to Various Synthetic Cannabinoids for Use in Fluorescence-Based Detection Platforms
Abstract
We have been able to synthesize and characterize several synthetic cannabinoid-dark quencher (DQ) conjugates for the use in fluorescence-based cannabinoid detection platforms. The carboxylic acid reactive QSY 7 amine was reacted with the carboxylated metabolites of JWH 018 and JWH 073 via a simple peptide conjugation reaction with the catalyst O-benzotriazole-N,N,N',N'-tetramethyl-uronium-hexafluoro-phosphate to yield 3 new DQ conjugates. Thin layer chromatography and liquid chromatography coupled mass spectrometry show full conversion of the synthetic cannabinoid starting material to product with observed and expected masses of 1,097 g/mol for JWH 018 pentanoic acid QSY 7 amine and 1,081.4 g/mol for JWH 073 butanoic acid-QSY 7 amine. These characterized conjugates are now ready for cannabinoid-receptor-based binding assays.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2015
- Accession Number
- ADA613664
Entities
People
- Abby L. West
- Joseph Dougherty
- Mark H Griep
- Nabila Hoque
- Shashi P. Karna
Organizations
- United States Army Research Laboratory