Microvascularized 3D Breast Cancer Constructs for Drug Testing and Development
Abstract
The project plan is to develop a three-dimensional (3D) microvascularized in vitro model system for breast cancer that could be used to culture individual cancer cells from patients and permit testing of a panel of chemotherapeutics. The research project will develop a new approach for drug efficacy testing that can better predict in vivo responses to therapeutics than current in vitro preclinical models. There are two aims. In the first, a microphysiologic, microvascularized 3D breast cancer model system that incorporates breast cancer associated fibroblasts (CAFs), microvascular endothelial cells (ECs), and cancer epithelial cells, embedded in extracellular matrix (ECM) supported by a novel carbon foam (reticulate vitreous carbon [RVC]) scaffold that contains EC-lined microchannels, will be assembled using breast cancer cell lines and characterized. In Aim 2, epithelial and stromal cells from cancer tissues from patients will be incorporated into the model system and parameters adjusted to achieve a microscopic architecture and representative molecular subtype of the originating cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2014
- Accession Number
- ADA613716
Entities
People
- Joel L. Berry
Organizations
- University of Alabama