Characterization and Expression of Drug Resistance Genes in MDROs Originating from Combat Wound Infections
Abstract
Twenty-five multi-drug resistant organisms (MDROs) of high military importance were sequenced using the Illumina HiSeq platform to identify drug resistance and virulence mechanisms contributing to their success as pathogens in the wound environment. The genomes were annotated and submitted to GenBank. Novel bioinformatics pipelines have been developed to (1) identify highly variable genomic regions, (2) classify antibiotic resistance islands, and (3) determine diversity of gene clusters associated with virulence in A. baumannii. Three top priority MDROs and three commensal microbes were chosen and obtained from WRAIR MRSN and ATCC for in vitro co-culturing in confrontation assays aimed at investigating alterations in gene expression, particularly antibiotic resistance genes and virulence factors. Confrontation assays were also carried out to assess gene regulation when the MDROs were co-cultured with cultured human skin cells. RNA-seq analysis of the bacteria-bacteria and bacteria-human confrontation assays are currently in progress. Manuscripts are in preparation describing the findings from this study.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2014
- Accession Number
- ADA613721
Entities
People
- Agnes Chan
- Derrick E Fouts
Organizations
- J. Craig Venter Institute