Targeting Palmitoyl Acyltransferases in Mutant NRAS-Driven Melanoma

Abstract

Mutations in N-RAS are linked to ~20% of melanoma with no effective treatment. Targeting palmitoyl acyltansferases (PATs) involved in N-RAS regulation could be a novel strategy to treat N-RAS mutant melanoma. The objective of the project is to identify PATs responsible for NRAS activation in melanoma cells using chemical biology and functional genomic approaches. In the first year of the study, we have developed more potent chemical probes to profile PATs in cells, and have carried out PATs profiling in melanoma cells using chemical probes and mRNA profiling. We have identified candidate PATs highly expressed in NRAS melanoma cells. We have developed shRNA reagents to further study their functions.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2014
Accession Number
ADA613837

Entities

People

  • Xu Wu

Organizations

  • Massachusetts General Hospital

Tags

DTIC Thesaurus Topics

  • Biological Products
  • Biology
  • Biomedical Research
  • Cell Line
  • Cells
  • Chemistry
  • Health Services
  • Mass Spectrometry
  • Melanoma
  • Molecules
  • Neoplasms
  • Proteins
  • Proteomics
  • Regulations
  • Skin Cancer
  • Small Molecules
  • Targeting

Fields of Study

  • Biology

Readers

  • Canadian European Scientific Immigration and Epilepsy Clearance Studies
  • Molecular and genetic basis of cancer.
  • Oncology (Cancer Research).