A Novel Mechanism for the Pathogenesis of Nonmelanoma Skin Cancer Resulting from Early Exposure to Ultraviolet Light

Abstract

We proposed that skin egress may be a characteristic of skin stem cells in response to ultraviolet light. Despite the poor breeding characteristics of our transgenic mice, we made 3 significant findings: 1) The first of two skin grafting studies was performed Aug. 14th-28th. FACS data were collected and analyzed. Practice surgeries were performed to get technique down. A repetition of this study will be performed on October 2nd. 2) A small pilot DMBA/TPA study was performed to assess whether keratinocytes migrated from the epidermis during the early stages of skin tumor promotion. Tissues were collected and analyzed. A larger experiment with additional mice will be performed this Fall. 3) We demonstrated using our K14EGFP/TOM transgenic mice that a low but significant number of K14-expressing cells are found in the bone marrow. The significance of these findings is that: 1) skin egress might be a characteristic of skin stem cells in response to ultraviolet light, and 2) bone marrow may be a long-lived reservoir of sunlight initiated stem cells that can repopulate the skin even years later upon damage caused by petrochemicals, skin wounding, or physical, chemical, or thermal damage to the skin.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2014
Accession Number
ADA614400

Entities

People

  • Rebecca J. Morris

Organizations

  • University of Minnesota

Tags

DTIC Thesaurus Topics

  • Blood
  • Bone Marrow
  • Bones
  • Breeding
  • Cells
  • Department Of Defense
  • Epidermis
  • Epithelial Cells
  • Epithelium
  • Neoplasms
  • Reservoirs
  • Skin Cancer
  • Skin Grafts
  • Stem Cells
  • Surgery
  • Tissues
  • Wounds And Injuries

Readers

  • Oncology (Cancer Research).
  • Toxicology/Environmental Toxicology
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology