Imaging Prostate Cancer Microenvironment by collagen Hybridization
Abstract
We continue to derivatize and test the collagen mimetic peptides (CMPs) for imaging. We continue to work on: (1) Dual radio- and fluorescent labeling of CMPs retaining high-affinity and specificity for intact and digested collagen (type I) films; (2) Validation of dual-labeled CMPs that display high affinity and specificity for stromal collagens in frozen PCa xenografts; and, (3) Measurement of pharmacokinetics and in vivo imaging of dual-labeled CMPs in mouse subcutaneous PC-3 xenograft (and pancreatic xenograft) models. Efforts have been focused on achieving a biologically stable CMP with both gamma-emitting and fluorescent labels. We have employed several strategies utilizing the previously biologically validated, high affinity basic CMP peptide core, which may contain chelators, radioiodine moieties and fluorescent dyes. While the radiochemistry aspect of the project has proved challenging, we have made substantial progress biologically with the optical agents as detailed below.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2014
- Accession Number
- ADA614415
Entities
People
- Martin G. Pomper
Organizations
- Johns Hopkins University