A Novel Tumor Antigen and Foxp3 Dual-Targeting Tumor Cell Vaccine Enhances the Immunotherapy in a Murine Model of Renal Cell Carcinoma

Abstract

During the past year I have generated Foxp3-over expressing RENCA cells, as the source of candidate dual targeting tumor cells vaccine. We have performed controlled vaccine therapy in RENCA model in three different schedules. When applied in a pre-vaccine schedule, RENCA and RENCA Foxp3 tumor cell vaccine efficiently prevented or inhibited orthotopic tumor growth. In an intervention schedule setting, RENCA Foxp3 tumor cell vaccine was superior to the RENCA tumor cell vaccine, and significantly reduced tumor growth, as compared to the vehicle and RENCA vaccine treated groups. In addition, the RENCA Foxp3 vaccine had activity to reduce number of T regulatory cells (Tregs) in tumor infiltrates and reduce Foxp3 expression level in Tregs. Our result support testing of tumor cell vaccine for Renal cell carcinoma in a clinical settings and also suggest the tumor and Tregs dual targeting vaccine may have better success rate in patients with established disease.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2014
Accession Number
ADA615157

Entities

People

  • Shen Li

Organizations

  • Health Research, Incorporated

Tags

DTIC Thesaurus Topics

  • Biological Staining And Labeling
  • Biomedical Research
  • Blood
  • Cells
  • Data Analysis
  • Diseases And Disorders
  • Intervention
  • Kidney Diseases
  • Luminescence
  • Lymphocytes
  • Macrophages
  • Myeloid Cells
  • Neoplasms
  • Statistical Analysis
  • T Lymphocytes
  • Targeting
  • Therapy

Fields of Study

  • Biology

Readers

  • Immunology
  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech