Development of a Diagnostic Tool to Detect DNA Methylation Biomarkers for Early-Stage Lung Cancer

Abstract

Biomarkers based on DNA methylation patterns combine genetic (inherited) and epigenetic (environmental) manifestations of lung-cancer risk and have great promise in detecting early-stage lung cancer. The current approaches for finding novel methylation targets, however, either do not provide DNA base-pair resolution or are laborious to perform. This application overcomes these challenges by combining protein engineering and fluorescence spectroscopy to develop a highly sensitive molecular complex for mapping DNA methylation patterns. We have successfully produced the proposed molecular probes. The performance of the DNA methylation-recognition domain and our detection platform has been successfully validated. The developed probe is ready to be transitioned to mapping DNA methylation patterns within a gene locus. This technology will facilitate clinical screening for early-stage lung cancer patients.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2015
Accession Number
ADA615421

Entities

People

  • Agnes Mendonca
  • Charny-yun Lin
  • Chongli Yuan
  • Julie C Liu
  • Renay Su
  • Seong-eun Kim

Organizations

  • Purdue University

Tags

DTIC Thesaurus Topics

  • Biological Markers
  • Biomedical Research
  • Biosensors
  • Biotechnology
  • Cancer
  • Detection
  • Engineering
  • Fluorescence
  • Genetic Structures
  • Lung Cancer
  • Neoplasms
  • Platforms
  • Protein Engineering
  • Recognition
  • Spectroscopy
  • Standards
  • Students

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Molecular and genetic basis of cancer.
  • Systems Analysis and Design

Technology Areas

  • Biotechnology