Time-Dependent Effects of Chlorhexidine Soaks on Grossly Contaminated Bone

Abstract

Objective: The purpose of this study was to quantify the reduction in the bacterial burden of grossly contaminated bone segments using different chlorhexidine (CHL) solutions. We hypothesized that 4% CHL would be the most efficient decontaminate. Methods: Fifty four bone segments were harvested from fresh frozen porcine legs. Each specimen was dropped onto a Mueller Hinton medium that was inoculated with Staphylococcus aureus (lux). These genetically engineered bacteria emit photons in propor tion to their number, allowing for quantification. The segments were retrieved after 5 seconds of exposure. Baseline imaging provided the initial bacterial load. An equal number of specimens were soaked in normal saline (NS), 2%CHL, or 4%CHL. Specimen reimaging was completed at the 5 , 10 , 20 , 30 , and 60 minute marks.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2012
Accession Number
ADA616144

Entities

People

  • Brendan D. Masini
  • Chad A. Krueger
  • Daniel J. Stinner
  • Joseph C Wenke
  • Joseph R. Hsu

Organizations

  • United States Army Institute of Surgical Research

Tags

DTIC Thesaurus Topics

  • Allografts
  • Analysis Of Variance
  • Bacteria
  • Biological Sciences
  • Case Studies
  • Contamination
  • Decontamination
  • Department Of Defense
  • Infection
  • Soft Tissues
  • Staphylococcus Aureus
  • Statistical Analysis
  • Time Intervals
  • Tissues
  • Transplants
  • United States
  • Viability

Readers

  • Exercise and Sports Science.
  • Mathematics or Statistics
  • Microbial Pathology

Technology Areas

  • Biotechnology