Research in Prevention and Treatment of Noise-Induced Hearing Loss (NIHL)
Abstract
The current study will identify D-methionine (D-met) dose and delayed time otoprotective response from steady state and impulse noise-induced hearing loss (NIHL). The third year was dedicated to completing data analysis from Year 2 and to further define maximum time delay for D-met post-noise rescue protection from permanent NIHL and to determine if additional D-met dosing for another 48 hours can improve post-noise rescue protection at the first rescue time with suboptimal protection. Groups of Chinchillas laniger (n = 10) received five intraperitoneal D-met (200 mg/kg/dose) every 12 hours beginning 28 or 32 hours after steady state or impulse. D-met otoprotection was assessed by auditory brainstem response (ABR) analysis and outer hair cell (OHC) quantification. ABR analyses identified 28 hour rescue as the first sub-optimal rescue dose time. Additional D-met administration at 28 hour rescue significantly recovered the lost protection in steady state noise-exposed animals. Histological cytocochleogram analysis is still in progress to confirm electrophysiological assessment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2015
- Accession Number
- ADA616584
Entities
People
- Kathleen Campbell
Organizations
- Southern Illinois University School of Medicine