Evaluation of a Perforated Drug Delivery System in Mice for Prolonged and Constant Release of a Hydrophilic Drug
Abstract
A drug delivery system (DDS) consisting of a perforated microtube (polyimide, inside diameter= 1.8 mm, tube length= 20 mm, hole size= 0.15 mm) was characterized in vitro and in vivo for its usefulness for long-term release of hydrophilic drugs at a constant rate. Sodium fluorescein mixed with stearic acid was used as the model drug. The DDS was packed with sodium fluorescein and stearic acid in ratios of 50:50, 40:60 and 25:75, respectively, and in vitro drug release studies were performed in saline. Linear release rates with R(expn 2) >0.9700 were obtained for all groups. Release rates of 1,077.3+/-264.6, 342.6+/-146.4, and 14.4+/-7.0 micro g/day for sodium fluorescein were obtained from the three groups respectively. After monitoring the in vitro release of fluorescein for 11 days, 7 tubes from the 40:60 group were implanted subcutaneously in each individual mice to study the in vivo release of fluorescein from the tubes by measuring the fluorescein in the urine fir 84 days. An initial rapid release during the first 4 days was followed by a near zero order fluorescence from the tunes (R[expn 2]=0.9870). Following completion of the study, the DDSs were retrieved for histology. Morphological analysis indicated no clinical adverse reaction at the site of device implantation specific to the device. The DDS was found to be biocompatible and capable of long-term constant release of hydrophilic drugs such as sodium fluorescein.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 17, 2012
- Accession Number
- ADA618498
Entities
People
- Ashish Rastogi
- Phillip D. Bowman
- Salomon Stavchansky
Organizations
- United States Army Institute of Surgical Research