A PEGylated Fibrin-Based Wound Dressing with Antimicrobial and Angiogenic Activity

Abstract

Wounds sustained under battlefield conditions are considered to be contaminated and their initial treatment should focus on decreasing this contamination and thus reducing the possibility of infection. The early and aggressive administration of antimicrobial treatment starting with intervention on the battlefield has resulted in improved patient outcomes and is considered the standard of care. Chitosan microspheres (CSM) loaded with silver sulfadiazine (SSD) were developed via a novel water-in-oil emulsion technique to address this problem. The SSD-loaded spheres were porous with needle-like structures (attributed to SSD) that were evenly distributed over the spheres. The average particle size of the SSD CSM was 125 180 micro m with 76.50 2.8% drug entrapment. As a potential new wound dressing with angiogenic activity SSD CSM particles were impregnated in polyethylene glycol (PEGylated) fibrin gels. In vitro drug release studies showed that a burst release of 27.02% in 6 h was achieved, with controlled release for 72 h, with an equilibrium concentration of 27.7% (70 micro g). SSD CSM PEGylated fibrin gels were able to exhibit microbicidal activity at 125 and 100 micro gml -1 against Staphylococcus aureus and Pseudomonas aeruginosa, respectively. The in vitro vasculogenic activity of this composite dressing was shown by seeding adipose-derived stem cells (ASC) in SSD CSM PEGylated fibrin gels. The ASC spontaneously formed microvascular tube-like structures without the addition of any exogenous factors. This provides a method for the extended release of an antimicrobial drug in a matrix that may provide an excellent cellular environment for revascularization of infected wounds.

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Document Details

Document Type
Technical Report
Publication Date
Apr 13, 2011
Accession Number
ADA618958

Entities

People

  • Conor Mullens
  • David G. Baer
  • Laura J Suggs
  • Robert J Christy
  • Ryan S. Stowers
  • Shanmuganathan Seetharaman
  • Shanmugasundaram Natesan

Organizations

  • United States Army Institute of Surgical Research

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Bacteria
  • Biomedical And Dental Materials
  • Burns
  • Chemical Synthesis
  • Chemistry
  • Combat Injuries
  • Dermatologic Agents
  • Electron Microscopes
  • Electron Microscopy
  • Health Services
  • Infection
  • Materials Science
  • Medical Personnel
  • Microscopes
  • Wound Infections

Readers

  • Immunology and Pathology
  • Microbial Pathology
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology