Proof-of-Concept Study: Novel Microbially-Driven Fenton Reaction for In Situ Remediation of Groundwater Contaminated with 1,4-Dioxane, Tetrachloroethene (PCE) and Trichloroethene (TCE)

Abstract

A microbially-driven Fenton reaction was designed to autocatalytically generate hydroxyl (HO) radicals that degrade 1,4-dioxane, TCE, and PCE. In comparison to conventional (purely abiotic) Fenton reactions, the microbially-driven Fenton reaction operates at circumneutral pH and does not require addition of exogenous H2O2 or UV irradiation to regenerate Fe(II). The 1,4-dioxane, TCE, and PCE degradation process was driven by the Fe(III)-reducing facultative anaerobe Shewanella oneidensis. S. oneidensis batch cultures were provided with lactate, Fe(III), and either 1,4-dioxane, TCE or PCE and exposed to alternating aerobic and anaerobic conditions. During the aerobic phase, S. oneidensis reduced O2 to H2O2, while during the anaerobic phase, S. oneidensis reduced Fe(III) to Fe(II). During the aerobic-to-anaerobic transition period, the produced Fe(II) and H2O2 interacted chemically via the Fenton reaction to form Fe(III), OH- ion, and HO radicals which oxidatively degraded 1,4-dioxane, TCE, and PCE at potential source zone concentrations. Under optimal conditions, the microbially-driven Fenton reaction completely degraded 1,4-dioxane, TCE, and PCE in 53 h with optimal aerobic-anaerobic cycling frequencies of 3 h. Acetate and oxalate were detected as transient intermediates during the microbially-driven Fenton degradation of 1,4-dioxane, an indication that conventional and microbially-driven Fenton degradation processes may follow similar reaction pathways.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 17, 2014
Accession Number
ADA619416

Entities

People

  • Thomas J. Dichristina

Organizations

  • Georgia Tech

Tags

DTIC Thesaurus Topics

  • Acetonitrile
  • Advanced Oxidation Processes
  • Air
  • Air Flow
  • Cell Count
  • Compressed Air
  • Culture Techniques
  • Degradation
  • Flow
  • Frequency
  • Gas Flow
  • Groundwater
  • Organic Compounds
  • Oxidation
  • Rodents
  • Standards
  • Water

Fields of Study

  • Environmental science

Readers

  • Groundwater Contamination Remediation.
  • Organic Chemistry

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation