Identification of the Elusive Mammalian Enzyme Phospatidylcholine-Specific Phospholipase C
Abstract
The long-term purpose of the present proposal aims at establishing the role of the elusive mammalian protein, phosphatidycholinespecific phospholipase C (PC-PLC) in the inflammatory processes involved in progression of rheumatoid arthritis (RA). Thus, the main scopes of this proposal are: 1. to identify the PC-PLC gene and protein; and 2. to test PC-PLC involvement in production of TNF alpha by monocytes. Major findings. In these six months we have mostly worked toward the identification of PC-PLC (Aim 1). Similarly to the results we reported in the previous technical report for LPS, we found that also treatment with oxidized LDLs does not cause a reproducible activation of PC-PLC in HUVEC cells (contrary to results published by another group). Thus we have proceeded with the isolation and quality control for total RNA isolated from control HUVECs cells and from HUVECs stimulated with 20% serum at two different time points (conditions in which we have confirmed time and time again activation of PC-PLC) and in duplicate. If the quality control shows acceptable purity of RNA, we will proceed with collecting another set of samples and perform transcriptome analysis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2015
- Accession Number
- ADA619494
Entities
People
- Chiara Luberto
Organizations
- State University of New York