Role of Ca++ Influx via Epidermal TRP Ion Channels
Abstract
To benefit military veterans with amputations who suffer skin problems on their amputation stumps, this proposal describes mechanistic studies to pave the way for novel methods of improving skin barrier function at the residual limb-prosthetic interface. Signaling systems in skin will be modulated to increase barrier function, attenuate irritant dermatitis, and characterize the underlying signaling mechanisms so that they can become better targets for treatment. Progress in year 1 of the funding period is described in this Annual Progress Report. We gained all the necessary regulatory approvals from the Durham VA, Duke University IRB and the DoD, the latter late in the funding period. We set up experiments in primary skin cells for mechanical stress. Throughout the funding period we have modified and adjusted our customized apparatus for delivering mechanical stress to the cells. Measurement of skin capacitance works, as do assays to measure gene-expression. We have measured lactate dehydrogenase as a measure of cellular injury in mechanically-stressed human skin keratinocytes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2014
- Accession Number
- ADA620001
Entities
People
- Wolfgang B Liedtke
Organizations
- Duke University