Targeting the Immune System's Natural Response to Cell Death to Improve Therapeutic Response in Breast Cancers

Abstract

We have proposed experiments to test the hypothesis that MerTK-mediated efferocytosis by tumor associated macrophages (TAMs) is a major limitation to effective therapeutic responses, because efferocytosis of dying tumor cells drives production of wound-healing/Th2-like cytokines, limits anti-tumor immunity, and promotes tumor growth. Scope: Two Aims were proposed to test this hypothesis. The goal of Aim 1 was to determine if MerTKdirected efferocytosis modulates cytokine expression, leukocyte infiltration, and growth of mouse mammary tumors, specifically testing the hypothesis that loss of MerTK would impair efferocytosis of dying tumor cells by TAMs, thus limiting production of Th2/WH cytokines in the tumor microenvironment (TME), resulting in decreased tumor growth and metastasis. The goal of Aim 2 was to measure the impact of MerTK-directed efferocytosis on tumor reemergence in therapeutically treated breast cancers, specifically testing the hypothesis that loss of MerTK-directed efferocytosis in the TME will limit Th2/WH cytokines, thereby preventing immune tolerance and tumor regrowth.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2015
Accession Number
ADA620800

Entities

People

  • Rebecca S. Cook

Organizations

  • Vanderbilt University

Tags

DTIC Thesaurus Topics

  • Blood
  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Physiological Processes
  • Cells
  • Culture Media
  • Culture Techniques
  • Gene Expression
  • Immune System
  • Leukocytes
  • Lymphocytes
  • Macrophages
  • Neoplasms
  • Phagocytes
  • Proteins
  • Wound Healing

Readers

  • Aerospace Propulsion Engineering.
  • Immunology and Pathology
  • Oncology