Effect of Cell-seeded Hydroxyapatite Scaffolds on Rabbit Radius Bone Regeneration
Abstract
Highly porous hydroxyapatite (HA) scaffolds were developed as bone graft substitutes using a template coating process, characterized, and seeded with bone marrow- derived mesenchymal stem cells (BMSCs). To test the hypothesis that cell-seeded HA scaffolds improve bone regeneration, HA scaffolds without cell seeding (HA-empty), HA scaffolds with 1.5 X 10(exp4) BMSCs (HA-low), and HA scaffolds with 1.5 X 10(exp6) BMSCs (HA-high) were implanted in a 10-mm rabbit radius segmental defect model for 4 and 8 weeks. Three different fluorochromes were administered at 2, 4, and 6 weeks after implantation to identify differences in temporal bone growth patterns. It was observed from fluorescence histomorphometry analyses that an increased rate of bone infiltration occurred from 0 to 2 weeks (p less than 0.05) of implantation for the HA-high group (2.9 + or - 0.5 mm) as compared with HA-empty (1.8 + or - 0.8 mm) and HA-low (1.3 + or - 0.2 mm) groups. No significant differences in bone formation within the scaffold or callus formation was observed between all groups after 4 weeks, with a significant increase in bone regenerated for all groups from 4 to 8 weeks (28.4% across groups). Although there was no difference in bone formation within scaffolds, callus formation was significantly higher in HA-empty scaffolds (100.9 + or - 14.1 mm(exp3)) when compared with HA-low (57.8 6 7.3 mm(exp 3) ; p less than or equal to 0.003) and HA-high (69.2 + or 110.4 mm 3 ; p less than or equal to 0.02) after 8 weeks. These data highlight the need for a better understanding of the parameters critical to the success of cell-seeded HA scaffolds for bone regeneration.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 22, 2013
- Accession Number
- ADA620815
Entities
People
- B. M. Singleton
- C. R. Rathbone
- D. S. Oh
- J. C. Wenke
- J. L. Ong
- M. R. Appleford
- T. Guda
Organizations
- United States Army Institute of Surgical Research