Antibiofilm and Antimicrobial Efficacy of DispersinB (registered trademark)-KSL-W Peptide-Based Wound Gel Against Chronic Wound Infection Associated Bacteria
Abstract
The medical importance of bacterial biofilms has increased with the recognition of biofilms as one of the major contributors to the slow or non-healing chronic wounds such as diabetic foot ulcers, venous leg ulcers, and pressure ulcers. Being a protected community of microorganisms, biofilms are notoriously refractory to antibiotic treatments. As the conventional treatment modalities have proven ineffective, this study provides the in vitro evidence to support the use of a novel combination of DispersinB antibiofilm enzyme that inhibits biofilm formation and disperses preformed biofilm, and thus making the biofilm bacteria more susceptible to a broad-spectrum KSL-W antimicrobial peptide. The combination of Silver-Sept(TM), DispersinB(registered trademark) and KSL-W(registered trademark) peptide showed synergistic antibiofilm and antimicrobial activity against chronic wound infection associated biofilm-embedded bacteria such as Methicillin- resistant Staphylococcus aureus (MRSA) ,Staphylococcus epidermidis, Coagulase-negative Staphylococci (CoNS), and Acinetobacter baumannii. In addition, the wound gel formulation comprising DispersinB, KSL-W peptide based-wound gel was significantly more effective in inhibiting the bio- film-embedded MRSA , S. epidermidis, CoNS, Vancomycin-resistant Enterococci, A. baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa (P less than 0.05). Thus, this study provides promising evidence for the potential application of antibiofilm-antimicrobial DispersinB(registered trademark) -KSL-W wound gel in chronic wound management.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 21, 2014
- Accession Number
- ADA620823
Entities
People
- Kai Poon Leung
- Purushottam V. Gawande
- Srinivasa Madhyastha
Organizations
- United States Army Institute of Surgical Research