A Genome-Wide Identified Risk Variant for PTSD is a Methylation Quantitative Trait Locus and Confers Decreased Cortical Activation to Fearful Faces
Abstract
Genetic factors appear to be highly relevant to predicting differential risk for the development of post-traumatic stress disorder (PTSD). In a discovery sample, we conducted a genome- wide association study (GWAS) for PTSD using a small military cohort (Systems Biology PTSD Biomarkers Consortium; SBPBC, N=147) that was designed as a case-controlled sample of highly exposed, recently returning veterans with and without combat-related PTSD. A genome-wide significant single nucleotide polymorphism (SNP), rs717947, at chromosome 4p15 (N=147, b=31.34, P=1.28^10 -8) was found to associate with the gold-standard diagnostic measure for PTSD (the Clinician Administered PTSD Scale). We conducted replication and follow-up studies in an external sample, a larger urban community cohort (Grady Trauma Project, GTP, N=2006), to determine the robustness and putative functionality of this risk variant. In the GTP replication sample, SNP rs717947 associated with PTSD diagnosis in females (N=2006, P=0.005), but not males. SNP rs717947 was also found to be a methylation quantitative trait locus (meQTL) in the GTP replication sample (N=157, P=0.002). Further, the risk allele of rs717947 was associated with decreased medial and dorsolateral cortical activation to fearful faces (N=53, P<0.05) in the GTP replication sample. These data identify a genome-wide significant polymorphism conferring risk for PTSD, which was associated with differential epigenetic regulation and with differential cortical responses to fear in a replication sample. These results may provide new insight into understanding genetic and epigenetic regulation of PTSD and intermediate phenotypes that contribute to this disorder.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 18, 2015
- Accession Number
- ADA621342
Entities
People
- Alicia K. Smith
- Duna Abu-amara
- Janine Flory
- Jennifer S. Stevens
- Kristina B. Mercer
- Lynn M. Almli
- Qian Meng
- Rasha Hammamieh
- Ruoting Yang
- Varun Kilaru
Organizations
- U.S. Army Center for Environmental Health Research